ASSAY METHOD THROUGH NON-BOILING DEGENERATION

Title:

ASSAY METHOD THROUGH NON-BOILING DEGENERATION

Document Type and Number:

Japanese Patent JPS58759

Kind Code:

A

Abstract:

Competitive protein binding radioassay for sera (or cell) vitamin B12 and/or serum folate (target components, or analytes) or other target components in liquid ambient utilizing a highly alkaline (pH 12-14) environment and reducing agent for denaturing separating the target component(s) from serum without boiling, consistent with other requirements of such assays, then reducing pH to an 8-10 range for effecting the competitive protein binding after which protein bound and unbound groups of radioactively tagged replicates of the target component(s) can be separated and detected to determine content of the target component(s) in the original serum (or cell), i.e. original endogenous analyte(s).

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Inventors:

RONARUDO REIMONDO FUORANDO
EDOWAADO SEA MENZU JIYUNIA

Application Number:

JP5024282A

Publication Date:

January 05, 1983

Filing Date:

March 30, 1982

Export Citation:

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Assignee:

AARU AI EI PURODAKUTSU INC

International Classes:

G01N33/60; G01N33/68; G01N33/82; (IPC1-7): G01N33/60; G01N33/68; G01N33/82

Attorney, Agent or Firm:

Wakabayashi Tadashi

Next Patent: SEPARATION OF CATABOLIC HEMOGLOBIN