Title:
ACID-RESISTANT YEAST STRAIN FOR HIGH-YIELD PRODUCTION OF L-MALIC ACID, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/093794
Kind Code:
A1
Abstract:
Disclosed is a genetically modified yeast strain for producing malic acid. The strain has the activity or an enhanced activity of a malic acid transporter and has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC 1.1.1.82); and also has the activity or an enhanced activity of at least one of the following: (i) a pyruvate carboxylase (EC 6.4.11); (ii) a phosphoenolpyruvate carboxykinase (EC 41.1.49); (iii) a phosphoenolpyruvate carboxylase; and (iv) a biotin transporter. Disclosed are a preparation method for the strain, a method for producing L-malic acid by means of using the strain, and the use thereof.
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Inventors:
ZHANG XUELI (CN)
FAN FEIYU (CN)
XI YONGYAN (CN)
XU HONGTAO (CN)
FAN FEIYU (CN)
XI YONGYAN (CN)
XU HONGTAO (CN)
Application Number:
PCT/CN2022/133964
Publication Date:
June 01, 2023
Filing Date:
November 24, 2022
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/00; C12N1/16; C12P7/46
Foreign References:
| US20190225947A1 | 2019-07-25 | |||
| EP2495304A1 | 2012-09-05 | |||
| CN101365782A | 2009-02-11 | |||
| CN102597224A | 2012-07-18 | |||
| CN106222099A | 2016-12-14 | |||
| CN113249238A | 2021-08-13 |
Other References:
SINGLETON ET AL.: "DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY", 1994, J. WILEY & SONS
SAMBROOK ET AL.: "MOLECULAR CLONING, A LABORATORY MANUAL", 1989, COLD SPRINGS HARBOR PRESS
"Uniprot", Database accession no. Q2UGL1
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 443
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
XI, YZHAN, T.XU, H.CHEN, J.BI, C.FAN, FZHANG, X: "Characterization of JEN family carboxylate transporters from the acid-tolerant yeast Pichia kudriavzevii and their applications in succinate production", MICROB BIOTECHNOL, 2021, pages 1 - 18
"GenBank", Database accession no. MW296878.1
"NCBI", Database accession no. XP_029319985.1
SAMBROOK ET AL.: "MOLECULAR CLONING, A LABORATORY MANUAL", 1989, COLD SPRINGS HARBOR PRESS
"Uniprot", Database accession no. Q2UGL1
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 443
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
XI, YZHAN, T.XU, H.CHEN, J.BI, C.FAN, FZHANG, X: "Characterization of JEN family carboxylate transporters from the acid-tolerant yeast Pichia kudriavzevii and their applications in succinate production", MICROB BIOTECHNOL, 2021, pages 1 - 18
"GenBank", Database accession no. MW296878.1
"NCBI", Database accession no. XP_029319985.1
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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