Title:
ADHERENT CELL CULTURE SUBSTRATE, CULTURE VESSEL, PEELING METHOD OF CELL, AND METHOD FOR MANUFACTURING ADHERENT CELL CULTURE SUBSTRATE
Document Type and Number:
WIPO Patent Application WO/2021/079861
Kind Code:
A1
Abstract:
The present invention can mass-culture adherent cells and make it possible to peel the adherent cells from a culture vessel. A sheet-shaped substrate used to manufacture a culture vessel for adherent cells is used as an adherent cell culture substrate which has, at one surface side of the substrate, a groove including a crest-shaped part and a valley-shaped part, and in which the distance from the top end of the crest-shaped part to the other surface of the substrate is 1 mm or less. In addition, it is preferable that a cross-section vertical to the direction in which the groove extends is provided in an approximately V-shape and an inclination angle of the side surface of the approximately V-shape at the groove is 80 degrees or less. Furthermore, it is preferable that a plurality of grooves are provided in a straight line shape, and the top end of the crest-shape part has a straight line shape.
Inventors:
KOSEKI OSAMU (JP)
TANAKA SATOSHI (JP)
MATSUOKA YOSUKE (JP)
NISHIYAMA TAKAHARU (JP)
TANAKA SATOSHI (JP)
MATSUOKA YOSUKE (JP)
NISHIYAMA TAKAHARU (JP)
Application Number:
PCT/JP2020/039337
Publication Date:
April 29, 2021
Filing Date:
October 20, 2020
Export Citation:
Assignee:
TOYO SEIKAN GROUP HOLDINGS LTD (JP)
International Classes:
C12M1/00; C12N5/071; C12N5/10
Foreign References:
| US4939151A | 1990-07-03 | |||
| JP2006502408A | 2006-01-19 | |||
| JP2003503715A | 2003-01-28 | |||
| JPH0638734A | 1994-02-15 |
Other References:
WANG, PENG-YUAN ET AL.: "Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering", COLLOIDS AND SURFACES B: BIOINTERFACES, vol. 110, 2013, pages 88 - 95, XP028573113, DOI: 10.1016/j.colsurfb.2013.04.016
Attorney, Agent or Firm:
IKUTOMI Shigekazu (JP)
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