Title:
ANTI-SENESCENT DRUG SCREENING METHOD
Document Type and Number:
WIPO Patent Application WO/2023/241118
Kind Code:
A1
Abstract:
The present invention relates to the technical field of genetic engineering, and in particular to an anti-senescent drug screening method. The screening method comprises: treating a GFP-progerin protein overexpressing cell by using a drug to be screened; comparing the treated GFP-progerin protein overexpressing cell with a GFP-progerin protein overexpressing cell that is not treated by the drug to be screened; and determining, according to a change in cell morphology, an anti-senescent function of the drug to be screened. The present invention finds that a progerin protein positioned on a nuclear membrane can cause a cell to show obvious characteristics of senescence, including nuclear membrane shrinkage and nuclear membrane budding. However, a progerin protein positioned in a cell nucleus would not cause a cell to show obvious characteristics of senescence. If a drug has an anti-senescent effect, a progerin protein positioned on a nuclear membrane would be transferred into a cell nucleus. On this basis, the present invention provides an anti-senescent drug screening method, capable of accurately and efficiently screening out an anti-senescent drug.
Inventors:
ZHANG CHUANMAO (CN)
WANG XIANGYANG (CN)
WANG XIANGYANG (CN)
Application Number:
PCT/CN2023/080293
Publication Date:
December 21, 2023
Filing Date:
March 08, 2023
Export Citation:
Assignee:
UNIV BEIJING (CN)
International Classes:
C12Q1/02
Domestic Patent References:
| WO2020061497A1 | 2020-03-26 |
Foreign References:
| CN115044639A | 2022-09-13 | |||
| CN114958766A | 2022-08-30 | |||
| US20140039010A1 | 2014-02-06 |
Other References:
PAN, XUMENG: "The role of accumulation of prelamin A in premature aging and related mechanisms", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 15 January 2021 (2021-01-15)
HU XIAO-TING, LIU XIN-GUANG, CHEN WEI-CHUN: "Mechanism of Genomic Instability Induced by Progerin", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 35, no. 8, 1 January 2019 (2019-01-01), pages 843 - 849, XP093119033, DOI: 10.13865/j.cnki.cjbmb.2019.08.09
GAO, ZHANJUAN ET AL.: "Research on the progress of relationships between LMNA products and aging or age-related diseases", CHINESE JOURNAL OF GERIATRICS, CHINESE MEDICAL ASSOCIATION, CN, vol. 33, no. 11, 14 November 2014 (2014-11-14), CN, pages 1247 - 1250, XP009551268, ISSN: 0254-9026
LÓPEZ-OTÍN CARLOS; BLASCO MARIA A.; PARTRIDGE LINDA; SERRANO MANUEL; KROEMER GUIDO : "The Hallmarks of Aging", CELL, ELSEVIER, AMSTERDAM NL, vol. 153, no. 6, 27 May 2013 (2013-05-27), Amsterdam NL , pages 1194 - 1217, XP028563547, ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.05.039
HU XIAO-TING, LIU XIN-GUANG, CHEN WEI-CHUN: "Mechanism of Genomic Instability Induced by Progerin", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 35, no. 8, 1 January 2019 (2019-01-01), pages 843 - 849, XP093119033, DOI: 10.13865/j.cnki.cjbmb.2019.08.09
GAO, ZHANJUAN ET AL.: "Research on the progress of relationships between LMNA products and aging or age-related diseases", CHINESE JOURNAL OF GERIATRICS, CHINESE MEDICAL ASSOCIATION, CN, vol. 33, no. 11, 14 November 2014 (2014-11-14), CN, pages 1247 - 1250, XP009551268, ISSN: 0254-9026
LÓPEZ-OTÍN CARLOS; BLASCO MARIA A.; PARTRIDGE LINDA; SERRANO MANUEL; KROEMER GUIDO : "The Hallmarks of Aging", CELL, ELSEVIER, AMSTERDAM NL, vol. 153, no. 6, 27 May 2013 (2013-05-27), Amsterdam NL , pages 1194 - 1217, XP028563547, ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.05.039
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (CN)
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