Title:
GLUCOSE DEHYDROGENASE HAVING ALTERED PROPERTIES
Document Type and Number:
WIPO Patent Application WO/2010/137489
Kind Code:
A1
Abstract:
Disclosed is a glucose dehydrogenase (GDH) which has high thermal stability and high substrate specificity and can exhibit a high affinity for a substrate when nicotinamide adenine dinucleotide (NAD) is used as a coenzyme. A GDH having high thermal stability and strict substrate specificity can be obtained from hyperthermophilic archaea Thermoproteus sp. strain GDH1. Further, a modified GDH which can exhibit an improved affinity for glucose when NAD is used as a coenzyme can be successfully produced by substituting an amino acid residue located at a specific position in the aforementioned GDH by a different amino acid residue.
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Inventors:
AIBA HIROSHI (JP)
KITABAYASHI MASAO (JP)
NISHIYA YOSHIAKI (JP)
KITABAYASHI MASAO (JP)
NISHIYA YOSHIAKI (JP)
Application Number:
PCT/JP2010/058332
Publication Date:
December 02, 2010
Filing Date:
May 18, 2010
Export Citation:
Assignee:
TOYO BOSEKI (JP)
AIBA HIROSHI (JP)
KITABAYASHI MASAO (JP)
NISHIYA YOSHIAKI (JP)
AIBA HIROSHI (JP)
KITABAYASHI MASAO (JP)
NISHIYA YOSHIAKI (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12P21/02; G01N27/327; G01N27/416; G01N33/66
Domestic Patent References:
| WO2009087929A1 | 2009-07-16 |
Other References:
SEIBERS, B. ET AL.: "Carbohydrate metabolism in Thermoproteus tenax: in vivo utilization of the non-phosphorylative Entner-Doudoroff pathway and characterization of its first enzyme, glucose dehydrogenase.", ARCH. MICROBIOL., vol. 168, no. 2, April 1997 (1997-04-01), pages 120 - 127, XP008130269, DOI: doi:10.1007/s002030050477
SEIBERS, B. ET AL.: "Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data.", J. BACTERIOL., vol. 186, no. 7, April 2004 (2004-04-01), pages 2179 - 2194
SEIBERS, B. ET AL.: "Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data.", J. BACTERIOL., vol. 186, no. 7, April 2004 (2004-04-01), pages 2179 - 2194
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