Title:
IMMOBILIZED ENDOGLYCOSIDASE FUSION PROTEIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/002330
Kind Code:
A1
Abstract:
A method for preparing an antibody-drug conjugate. The antibody-drug conjugate is formed by means of site-directed conjugation on the basis of an N-glycosylation site of an Fc region of an antibody. The method comprises: (1) providing a donor containing an oxazoline oligosaccharide, an antibody containing a GlcNAc motif, and an immobilized endoglycosidase having a glycoside transfer activity; and (2) covalently linking the activated donor containing the oxazoline oligosaccharide to the antibody containing the GlcNAc motif by means of the catalytic action of the endoglycosidase; therefore, one-step conjugation is realized. The present invention also relates to an endoglycosidase fusion protein, which comprises a covalently linked endoglycosidase, a Halo tag, and/or a His tag. The present invention also relates to an immobilized endoglycosidase fusion protein obtained by means of immobilizing an endoglycosidase fusion protein on a support, a prepacked column filled with the immobilized fusion protein, and a method for purifying an antibody conjugate by using the immobilized endoglycosidase fusion protein.
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Inventors:
LV CAO (CN)
YUAN JINDUO (CN)
QIN GANG (CN)
XIONG MEIJUN (CN)
YUAN JINDUO (CN)
QIN GANG (CN)
XIONG MEIJUN (CN)
Application Number:
PCT/CN2023/104549
Publication Date:
January 04, 2024
Filing Date:
June 30, 2023
Export Citation:
Assignee:
GENEQUANTUM HEALTHCARE SUZHOU CO LTD (CN)
International Classes:
C07K16/00; A61K47/68; C12M1/40; C12N11/00
Domestic Patent References:
| WO2018036403A1 | 2018-03-01 |
Foreign References:
| CN104220603A | 2014-12-17 | |||
| US20120226024A1 | 2012-09-06 | |||
| CN114395051A | 2022-04-26 | |||
| CN114480115A | 2022-05-13 | |||
| CN109071630A | 2018-12-21 |
Other References:
SHI WEI, LI WANZHEN, ZHANG JIANXIN, LI TIEHAI, SONG YAKAI, ZENG YUE, DONG QIAN, LIN ZENG, GONG LIKUN, FAN SHUQUAN, TANG FENG, HUAN: "One-step synthesis of site-specific antibody–drug conjugates by reprograming IgG glycoengineering with LacNAc-based substrates", ACTA PHARMACEUTICA SINICA B, vol. 12, no. 5, 1 May 2022 (2022-05-01), pages 2417 - 2428, XP093124704, ISSN: 2211-3835, DOI: 10.1016/j.apsb.2021.12.013
CHUANG, H.Y. ET AL.: "Development of Biotinylated and Magnetic Bead-Immobilized Enzymes for Efficient Glyco-Engineering and Isolation of Antibodies", BIOORGANIC CHEMISTRY, vol. 112, 26 March 2021 (2021-03-26), XP086605807, DOI: 10.1016/j.bioorg.2021.104863
CHUANG, H.Y. ET AL.: "Development of Biotinylated and Magnetic Bead-Immobilized Enzymes for Efficient Glyco-Engineering and Isolation of Antibodies", BIOORGANIC CHEMISTRY, vol. 112, 26 March 2021 (2021-03-26), XP086605807, DOI: 10.1016/j.bioorg.2021.104863
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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