Title:
LIGHT-RESPONSIVE MODIFIED OPSIN
Document Type and Number:
WIPO Patent Application WO/2024/048688
Kind Code:
A1
Abstract:
The present disclosure provides an opsin that has a light cycle characteristic. Specifically, the present disclosure provides a protein including an amino acid sequence of an opsin, wherein the amino acid sequence of the opsin includes a modification of the amino acid corresponding to position 188 when aligned with SEQ ID NO:1. The present disclosure also provides a medicine, preparation, composition, treatment method, and the like that include said protein and/or a nucleic acid molecule, a nucleic acid construct, and/or cells related to said protein.
Inventors:
YAMASHITA TAKAHIRO (JP)
SAKAI KAZUMI (JP)
SHICHIDA YOSHINORI (JP)
SATO KEITA (JP)
AOKI SHION (JP)
KATADA YUSAKU (JP)
SAKAI KAZUMI (JP)
SHICHIDA YOSHINORI (JP)
SATO KEITA (JP)
AOKI SHION (JP)
KATADA YUSAKU (JP)
Application Number:
PCT/JP2023/031644
Publication Date:
March 07, 2024
Filing Date:
August 30, 2023
Export Citation:
Assignee:
UNIV KYOTO (JP)
RESTORE VISION INC (JP)
RESTORE VISION INC (JP)
International Classes:
C12N15/12; A61K35/12; A61K38/16; A61K48/00; A61P27/02; C07K14/47; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Domestic Patent References:
| WO2020206098A1 | 2020-10-08 |
Other References:
SAKAI KAZUMI, SHICHIDA YOSHINORI, IMAMOTO YASUSHI, YAMASHITA TAKAHIRO: "Creation of photocyclic vertebrate rhodopsin by single amino acid substitution", BIORXIV, 6 December 2021 (2021-12-06), XP093143365, Retrieved from the Internet [retrieved on 20240320], DOI: 10.1101/2021.12.06.471433
YOSHIDA KAZUHO, TSUNODA SATOSHI P., BROWN LEONID S., KANDORI HIDEKI: "A unique choanoflagellate enzyme rhodopsin exhibits light-dependent cyclic nucleotide phosphodiesterase activity", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 292, no. 18, 1 March 2017 (2017-03-01), US , pages 7531 - 7541, XP093143366, ISSN: 0021-9258, DOI: 10.1074/jbc.M117.775569
KEITA SATO, TAKAHIRO YAMASHITA, HIDEYO OHUCHI, ATSUKO TAKEUCHI, HITOSHI GOTOH, KATSUHIKO ONO, MISAO MIZUNO, YASUHISA MIZUTANI, SAY: "Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor", NATURE COMMUNICATIONS, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055704703, DOI: 10.1038/s41467-018-03603-3
SUNGSM CHING-HWA, DAVENPORTSB CAROL M, NATHANSSBN JEREMY: "Rhodopsin Mutations Responsible for Autosomal Dominant Retinitis Pigmentosa", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 35, 15 December 1993 (1993-12-15), pages 26646 - 26649, XP093143371
YOSHIDA KAZUHO, TSUNODA SATOSHI P., BROWN LEONID S., KANDORI HIDEKI: "A unique choanoflagellate enzyme rhodopsin exhibits light-dependent cyclic nucleotide phosphodiesterase activity", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 292, no. 18, 1 March 2017 (2017-03-01), US , pages 7531 - 7541, XP093143366, ISSN: 0021-9258, DOI: 10.1074/jbc.M117.775569
KEITA SATO, TAKAHIRO YAMASHITA, HIDEYO OHUCHI, ATSUKO TAKEUCHI, HITOSHI GOTOH, KATSUHIKO ONO, MISAO MIZUNO, YASUHISA MIZUTANI, SAY: "Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor", NATURE COMMUNICATIONS, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055704703, DOI: 10.1038/s41467-018-03603-3
SUNGSM CHING-HWA, DAVENPORTSB CAROL M, NATHANSSBN JEREMY: "Rhodopsin Mutations Responsible for Autosomal Dominant Retinitis Pigmentosa", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 35, 15 December 1993 (1993-12-15), pages 26646 - 26649, XP093143371
Attorney, Agent or Firm:
KAMADA, Mitsunori et al. (JP)
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