Title:
METHOD FOR CHARACTERISING POLYPEPTIDES BY MALDI-TOF MAS SPECTROMETRY
Document Type and Number:
WIPO Patent Application WO2005012914
Kind Code:
A3
Abstract:
Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte; wherein the light absorbing label comprises a fluorophore moiety, and wherein prior to detecting by mass spectrometry, the analyte is selected for detection on the basis of its fluorophore moiety.
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Inventors:
SCHAFER JURGEN (DE)
HAMON CHRISTIAN (DE)
SCHWARZ JOSEF (DE)
PEARCE CHRISTOPHER (GB)
HAMON CHRISTIAN (DE)
SCHWARZ JOSEF (DE)
PEARCE CHRISTOPHER (GB)
Application Number:
PCT/GB2004/003139
Publication Date:
June 30, 2005
Filing Date:
July 22, 2004
Export Citation:
Assignee:
XZILLION GMBH & CO KG (DE)
PROTEOME SCIENCES PLC (GB)
SCHAFER JURGEN (DE)
HAMON CHRISTIAN (DE)
SCHWARZ JOSEF (DE)
PEARCE CHRISTOPHER (GB)
PROTEOME SCIENCES PLC (GB)
SCHAFER JURGEN (DE)
HAMON CHRISTIAN (DE)
SCHWARZ JOSEF (DE)
PEARCE CHRISTOPHER (GB)
International Classes:
C07D403/14; C07D413/14; C07K1/13; G01N33/533; G01N33/68; (IPC1-7): G01N33/68; C07D311/82; C07K1/13; C09B23/00; G01N33/533
Domestic Patent References:
| WO2002042427A2 | 2002-05-30 | |||
| WO2002099435A1 | 2002-12-12 | |||
| WO2003005026A2 | 2003-01-16 | |||
| WO2000002895A1 | 2000-01-20 |
Foreign References:
| US20020182651A1 | 2002-12-05 | |||
| US20030077595A1 | 2003-04-24 | |||
| US5512667A | 1996-04-30 |
Other References:
AEBERSOLD R ET AL: "Mass spectrometry in proteomics.", CHEMICAL REVIEWS. FEB 2001, vol. 101, no. 2, February 2001 (2001-02-01), pages 269 - 295, XP002309029, ISSN: 0009-2665
JUNGBLUT P ET AL: "PROTEIN IDENTIFICATION FROM 2-DE GELS BY MALDI MASS SPECTROMETRY", MASS SPECTROMETRY REVIEWS, JOHN WILEY AND SONS, NEW YORK, NY, US, vol. 16, 1997, pages 145 - 162, XP001023942, ISSN: 0277-7037
MUJUMDAR R B ET AL: "CYANINE DYE LABELING REAGENTS: SULFOINDOCYANINE SUCCINIMIDYL ESTERS", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 4, no. 2, 1 March 1993 (1993-03-01), pages 105 - 111, XP000654181, ISSN: 1043-1802
JUNGBLUT P ET AL: "PROTEIN IDENTIFICATION FROM 2-DE GELS BY MALDI MASS SPECTROMETRY", MASS SPECTROMETRY REVIEWS, JOHN WILEY AND SONS, NEW YORK, NY, US, vol. 16, 1997, pages 145 - 162, XP001023942, ISSN: 0277-7037
MUJUMDAR R B ET AL: "CYANINE DYE LABELING REAGENTS: SULFOINDOCYANINE SUCCINIMIDYL ESTERS", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 4, no. 2, 1 March 1993 (1993-03-01), pages 105 - 111, XP000654181, ISSN: 1043-1802
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