Title:
METHOD FOR DETECTING MUTATIONS IN TARGET BASE SEQUENCE OF NUCLEIC ACID, METHOD FOR SELECTIVELY INHIBITING AMPLIFICATION OF NUCLEIC ACID, AND KIT FOR IMPLEMENTING SAME
Document Type and Number:
WIPO Patent Application WO/2023/106200
Kind Code:
A1
Abstract:
The present invention has the objective of providing: a convenient method capable of detecting mutant genes at mutant gene prevalences which are lower than the detection limit of mutant genes when using PCR clamping; and a kit for implementing the same. The present invention also has the objective of providing: a convenient method capable of selectively inhibiting amplification of target base sequences in nucleic acid amplification reactions; and a kit for implementing the same. A nucleic acid clamp is caused to be concomitantly present, during a nucleic acid amplification reaction, with an amphoteric copolymer which has a cationic structural unit (1) containing an amino acid and an anionic structural unit (2), the mole ratio of the cationic structural unit (1) to the anionic structural unit (2) (cationic structural unit (1)/anionic structural unit (2)) being in the range of 0.13 to 1.62, thereby enhancing the amplification inhibition, by the nucleic acid clamp, of nucleic acids having base sequences complementary thereto.
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Inventors:
FUJIMURA NAHOHIRO (JP)
TACHIBANA AMI (JP)
UCHIKI TOMOAKI (JP)
TAKEUCHI MINORU (JP)
TERUUCHI YOKO (JP)
WATANABE KOJI (JP)
TACHIBANA AMI (JP)
UCHIKI TOMOAKI (JP)
TAKEUCHI MINORU (JP)
TERUUCHI YOKO (JP)
WATANABE KOJI (JP)
Application Number:
PCT/JP2022/044363
Publication Date:
June 15, 2023
Filing Date:
December 01, 2022
Export Citation:
Assignee:
NITTO BOSEKI CO LTD (JP)
International Classes:
C12Q1/686; C12Q1/6858
Domestic Patent References:
| WO2016167320A1 | 2016-10-20 | |||
| WO2003018841A1 | 2003-03-06 |
Foreign References:
| JP2004537263A | 2004-12-16 | |||
| JP2018104850A | 2018-07-05 |
Other References:
SAKAKI S, NAKABAYASHI N: "MPC polymer ni yoru 1 enki takata (SNPs) no kokateki na ninshiki / EFFECTIVE RECOGNITION OF SINGLE NUCLEOTIDE POLYMORPHISM (SNPs) BY USE OF MPC POLYMERS", POLYMER PREPRINTS. JAPAN, SOCIETY OF POLYMER SCIENCE., JP, vol. 50, no. 5, 1 May 2001 (2001-05-01), JP , pages 992, XP002960296
JIALUN HAN, WU JINCAI, DU JIE: "Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer", MOLECULES, SPRINGER VERLAG, BERLIN, DE, vol. 24, no. 3, DE , pages 575, XP055556584, ISSN: 1433-1373, DOI: 10.3390/molecules24030575
TACHIBANA AMI, FUJIMURA NAHOHIRO, TAKEUCHI MINORU, WATANABE KOJI, TERUUCHI YOKO, UCHIKI TOMOAKI: "Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands", BIOLOGY METHODS AND PROTOCOLS, vol. 7, no. 1, 10 January 2022 (2022-01-10), XP093068867, DOI: 10.1093/biomethods/bpac009
JIALUN HAN, WU JINCAI, DU JIE: "Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer", MOLECULES, SPRINGER VERLAG, BERLIN, DE, vol. 24, no. 3, DE , pages 575, XP055556584, ISSN: 1433-1373, DOI: 10.3390/molecules24030575
TACHIBANA AMI, FUJIMURA NAHOHIRO, TAKEUCHI MINORU, WATANABE KOJI, TERUUCHI YOKO, UCHIKI TOMOAKI: "Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands", BIOLOGY METHODS AND PROTOCOLS, vol. 7, no. 1, 10 January 2022 (2022-01-10), XP093068867, DOI: 10.1093/biomethods/bpac009
Attorney, Agent or Firm:
ASAMURA IP P.C. (JP)
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