Title:
METHOD FOR MODIFYING AMINO ACID ATTENUATOR AND USE OF SAME IN PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2018/077159
Kind Code:
A1
Abstract:
Disclosed is a method for modifying an amino acid attenuator, a type of amino acid attenuator mutants, engineered bacteria generated on the basis of the amino acid attenuator mutants, and use of the engineered bacteria. Further disclosed is a method for relieving the attenuation regulation of an amino acid operon gene. The amino acid operon gene is modified as follows: removing a gene coding for a leader peptide and an anterior reverse complementary palindromic sequence in the terminator stem-loop structure, and maintaining a posterior reverse complementary palindromic sequence in the terminator. The amino acid operon can be selected from histidine operon, tryptophan operon, phenylalanine operon, alanine operon, and threonine operon.
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Inventors:
WEN TINGYI (CN)
LIU SHUWEN (CN)
ZHANG YUN (CN)
SHANG XIULING (CN)
XIAO HAIHAN (CN)
LIU SHUWEN (CN)
ZHANG YUN (CN)
SHANG XIULING (CN)
XIAO HAIHAN (CN)
Application Number:
PCT/CN2017/107453
Publication Date:
May 03, 2018
Filing Date:
October 24, 2017
Export Citation:
Assignee:
INST MICROBIOLOGY CAS (CN)
International Classes:
C12N15/113; C12N1/21; C12N15/63; C12P13/06; C12P13/22; C12R1/19
Domestic Patent References:
| WO2003097839A1 | 2003-11-27 |
Foreign References:
| CN107058323A | 2017-08-18 | |||
| CN107236738A | 2017-10-10 | |||
| CN104204189A | 2014-12-10 |
Other References:
LIN WEIPING ET AL.: "Construction and expression of Ttp Operon Gene Mutant of E.coil", CHINESE JOURNAL OF BIOLOGICALS, vol. 23, no. 7, 20 July 2010 (2010-07-20), pages 711 - 713, ISSN: 1004-5503
ROESSER,JR. ET AL: "The effects of leader peptide sequence and length on attenuation control of the Trp operon of E.coil", NUCLEIC ACIDS RESEARCH, vol. 19, no. 4, 25 February 1991 (1991-02-25), pages 795 - 800, XP055599119, ISSN: 0305-1048, DOI: 10.1093/nar/19.4.795
"Molecular Cloning: A Laboratory Manual", SCIENCE PRESS
"Laboratory Experiments In Microbiology", HIGHER EDUCATION PRESS
GENE, vol. 226, no. 2, 1999, pages 297 - 305
ZHANG ET AL., PLOS GENETICS, vol. 8, no. 9, 2012, pages e1002987
AMINO ACIDS & BIOTIC RESOURCES, vol. 22, 2000, pages 59 - 60
See also references of EP 3533872A4
ROESSER,JR. ET AL: "The effects of leader peptide sequence and length on attenuation control of the Trp operon of E.coil", NUCLEIC ACIDS RESEARCH, vol. 19, no. 4, 25 February 1991 (1991-02-25), pages 795 - 800, XP055599119, ISSN: 0305-1048, DOI: 10.1093/nar/19.4.795
"Molecular Cloning: A Laboratory Manual", SCIENCE PRESS
"Laboratory Experiments In Microbiology", HIGHER EDUCATION PRESS
GENE, vol. 226, no. 2, 1999, pages 297 - 305
ZHANG ET AL., PLOS GENETICS, vol. 8, no. 9, 2012, pages e1002987
AMINO ACIDS & BIOTIC RESOURCES, vol. 22, 2000, pages 59 - 60
See also references of EP 3533872A4
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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