Title:
METHOD FOR SIMPLIFYING DESIGN, SYNTHESIS AND ASSEMBLY OF CHLOROPLAST GENOME OF CHLAMYDOMONAS REINHARDTII, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/026922
Kind Code:
A1
Abstract:
Provided are a method for simplifying the design, synthesis and assembly of a chloroplast genome of chlamydomonas reinhardtii, and use thereof. The method comprises: simplifying the design, total chemical synthesis, assembly, functional verification and use of the chloroplast genome of the chlamydomonas reinhardtii. On the basis of a natural organelle chloroplast genome of the chlamydomonas reinhardtii, a chloroplast genome of the chlamydomonas reinhardtii is re-designed and manually constructed. By means of deleting redundant genetic information, changing genetic elements or metabolic pathways or adding brand-new genetic elements or metabolic pathways, and the like, the genome is comprehensively modified; furthermore, design defects and sequence defects of the chloroplast genome of the chlamydomonas reinhardtii are repaired to achieve precise matching between the chemical synthesis and the design sequence, and verify and evaluate the design principle of the chloroplast genome of the chlamydomonas reinhardtii. On this basis, customized construction of chloroplasts is achieved, thereby providing brand-new chassis cells and optimized platform carriers for the research and production in fields such as medicine, energy, environment, and agriculture.
Inventors:
HU ZHANGLI (CN)
ZHANG GUIYING (CN)
JIA BIN (CN)
WANG CHAOGANG (CN)
JIANG YANAN (CN)
ZHANG GUIYING (CN)
JIA BIN (CN)
WANG CHAOGANG (CN)
JIANG YANAN (CN)
Application Number:
PCT/CN2022/112268
Publication Date:
February 08, 2024
Filing Date:
August 12, 2022
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C12N15/31; C12N1/13; C12N15/65; C12N15/81; C12N15/82; C12N15/90; C12P23/00; C12R1/89
Foreign References:
| CN101255438A | 2008-09-03 | |||
| CN1827769A | 2006-09-06 | |||
| US20090123977A1 | 2009-05-14 |
Other References:
LU YINHUA, XUE XIAOLI, LI LEI, QIN ZHONGJUN, JIANG WEIHONG: "Recent Advances in the Enabling Technologies for Synthetic Biology", SCIENCE CHINA: CHINESE BULLETIN OF LIFE SCIENCE = SCIENTIA SINICA VITAE, vol. 45, no. 10, 1 October 2015 (2015-10-01), pages 950 - 968, XP093119831, ISSN: 1674-7232, DOI: 10.1360/N052015-00048
KE ZHUFANG, XU XU-DONG; GAO HONG: "Unmarked Large Fragment Deletions in the Genome of Synechococcus Elongatus Pcc 7942 Based on Homologous Double-Crossover Recombination", SHUISHENG-SHENGWU-XUEBAO = ACTA HYDROBIOLOGICA SINICA, KEXUE CHUBANSHE, CHINA, vol. 46, no. 3, 31 March 2022 (2022-03-31), CHINA , pages 342 - 347, XP093135425, ISSN: 1000-3207, DOI: 10.7541/2022.2020.217
MURAKAMI, K. ET AL.: "Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism", APPL MICROBIOL BIOTECHNOL, vol. 75, 8 March 2007 (2007-03-08), XP019513683, DOI: 10.1007/s00253-007-0859-2
KE ZHUFANG, XU XU-DONG; GAO HONG: "Unmarked Large Fragment Deletions in the Genome of Synechococcus Elongatus Pcc 7942 Based on Homologous Double-Crossover Recombination", SHUISHENG-SHENGWU-XUEBAO = ACTA HYDROBIOLOGICA SINICA, KEXUE CHUBANSHE, CHINA, vol. 46, no. 3, 31 March 2022 (2022-03-31), CHINA , pages 342 - 347, XP093135425, ISSN: 1000-3207, DOI: 10.7541/2022.2020.217
MURAKAMI, K. ET AL.: "Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism", APPL MICROBIOL BIOTECHNOL, vol. 75, 8 March 2007 (2007-03-08), XP019513683, DOI: 10.1007/s00253-007-0859-2
Attorney, Agent or Firm:
JOHNSON INTELLECTUAL PROPERTY AGENCY(SHENZHEN) (CN)
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