Title:
METHOD FOR SYNTHESIS AND ASSEMBLY AND FUNCTIONAL TEST OF ARTIFICIAL CHLAMYDOMONAS REINHARDTII CHLOROPLAST GENOME
Document Type and Number:
WIPO Patent Application WO/2024/026923
Kind Code:
A1
Abstract:
Provided is a method for synthesis and assembly and functional test of artificial Chlamydomonas reinhardtii chloroplast genome. Full artificial synthesis of the Chlamydomonas reinhardtii chloroplast genome is proposed based on rational design of the Chlamydomonas reinhardtii chloroplast genome. Full chemical de novo synthesis and assembly of chloroplast genome are realized in a yeast-bacterial system by means of using fully chemically synthesized chloroplast genome fragments. The fully chemically synthesized chloroplast genome is then transfected into chlamydomonas cells to replace the original genome of the chloroplast. The chloroplast functions normally and is verified. Thus, the biological function of the fully chemically synthesized chloroplast genome is achieved. The Chlamydomonas reinhardtii chloroplast genome is demonstrated to be an efficient platform for carrying out synthetic biological operations, and the de novo design, the full-chemical synthesis, and in vitro assembly and identification of the genome provide new solutions for rational design and transformation and reconstruction of the photosynthesis system of photosynthetic organisms, improving the photosynthetic efficiency of crops, and dealing with agricultural crisis such as food security.
Inventors:
HU ZHANGLI (CN)
WANG CHAOGANG (CN)
GUO CHUNLI (CN)
ZHANG GUIYING (CN)
JIA BIN (CN)
WANG CHAOGANG (CN)
GUO CHUNLI (CN)
ZHANG GUIYING (CN)
JIA BIN (CN)
Application Number:
PCT/CN2022/112271
Publication Date:
February 08, 2024
Filing Date:
August 12, 2022
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C12N15/00; C12N1/13; C12N15/09; C12P21/00
Domestic Patent References:
| WO2009045550A2 | 2009-04-09 |
Foreign References:
| CN101939423A | 2011-01-05 | |||
| CN1827769A | 2006-09-06 | |||
| CN102630140A | 2012-08-08 | |||
| CN1807649A | 2006-07-26 | |||
| CN102146344A | 2011-08-10 | |||
| US20150329898A1 | 2015-11-19 |
Other References:
SIHAI HAN, HU ZHANGLI, WANG CHAOGANG: "Exppession of Foreign Genes in Chlamydomonas reinhardtii Chloroplast", BIOTECHNOLOGY BULLETIN, vol. 1, 26 February 2007 (2007-02-26), pages 89 - 94, XP093136285, DOI: 10.13560/j.cnki.biotech.bull.1985.2007.01.021
CHAO-GANG WANG, HU ZHANG-LI: "The research advance in nuclear transformation system of Chlamydomonas reinhardtii", JOURNAL OF ZHONGKAI AGROTECHNICAL COLLEGE, vol. 18, no. 2, 30 June 2005 (2005-06-30), pages 59 - 64, XP093136288
KÜCK ULRICH, SCHMITT OLGA: "The Chloroplast Trans-Splicing RNA–Protein Supercomplex from the Green Alga Chlamydomonas reinhardtii", CELLS, vol. 10, no. 2, 1 February 2021 (2021-02-01), pages 290, XP093136286, DOI: 10.3390/cells10020290
KATHIR PUSHPA, LAVOIE MATTHEW, BRAZELTON WILLIAM J., HAAS NANCY A., LEFEBVRE PAUL A., SILFLOW CAROLYN D.: "Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome", EUKARYOTIC CELL, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 2, no. 2, 1 April 2003 (2003-04-01), US , pages 362 - 379, XP093136290, ISSN: 1535-9778, DOI: 10.1128/EC.2.2.362-379.2003
CHAO-GANG WANG, HU ZHANG-LI: "The research advance in nuclear transformation system of Chlamydomonas reinhardtii", JOURNAL OF ZHONGKAI AGROTECHNICAL COLLEGE, vol. 18, no. 2, 30 June 2005 (2005-06-30), pages 59 - 64, XP093136288
KÜCK ULRICH, SCHMITT OLGA: "The Chloroplast Trans-Splicing RNA–Protein Supercomplex from the Green Alga Chlamydomonas reinhardtii", CELLS, vol. 10, no. 2, 1 February 2021 (2021-02-01), pages 290, XP093136286, DOI: 10.3390/cells10020290
KATHIR PUSHPA, LAVOIE MATTHEW, BRAZELTON WILLIAM J., HAAS NANCY A., LEFEBVRE PAUL A., SILFLOW CAROLYN D.: "Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome", EUKARYOTIC CELL, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 2, no. 2, 1 April 2003 (2003-04-01), US , pages 362 - 379, XP093136290, ISSN: 1535-9778, DOI: 10.1128/EC.2.2.362-379.2003
Attorney, Agent or Firm:
JOHNSON INTELLECTUAL PROPERTY AGENCY(SHENZHEN) (CN)
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