Title:
METHODS FOR PRODUCING EXTRACELLULAR VESICLES ENRICHED IN ANTI-INFLAMMATORY MICRORNAS
Document Type and Number:
WIPO Patent Application WO/2022/143784
Kind Code:
A1
Abstract:
Provided are methods of producing exosomes by culturing isolated stem cells with an effective amount of at least one immunoregulatory factor. The thus-produced exosomes are enriched in anti-inflammatory miRNAs and/or miRNAs that inhibits the epithelial-mesenchymal transition.
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Inventors:
LI PING (CN)
WANG JING (CN)
SHEN MEIPING (CN)
DAI CHENGXIANG (CN)
WANG JING (CN)
SHEN MEIPING (CN)
DAI CHENGXIANG (CN)
Application Number:
PCT/CN2021/142539
Publication Date:
July 07, 2022
Filing Date:
December 29, 2021
Export Citation:
Assignee:
CELLULAR BIOMEDICINE GROUP SHANGHAI LTD (CN)
International Classes:
A61K31/7105; A61K9/127; A61K35/28; A61K35/30; A61P37/00; A61P37/06; G01N33/50
Foreign References:
| US20200392219A1 | 2020-12-17 | |||
| US20160193252A1 | 2016-07-07 | |||
| US20190062710A1 | 2019-02-28 |
Other References:
GIUNTI DEBORA, MARINI CHIARA, PARODI BENEDETTA, USAI CESARE, MILANESE MARCO, BONANNO GIAMBATTISTA, KERLERO DE ROSBO NICOLE, UCCELL: "Role of miRNAs shuttled by mesenchymal stem cell-derived small extracellular vesicles in modulating neuroinflammation", SCIENTIFIC REPORTS, vol. 11, no. 1, 1 December 2021 (2021-12-01), pages 1 - 17, XP055948558, DOI: 10.1038/s41598-021-81039-4
PELTZER JULIETTE, LUND KYLE, GORIOT MARIE-EMMANUELLE, GROSBOT MARION, LATAILLADE JEAN-JACQUES, MAUDUIT PHILIPPE, BANZET SÉBASTIEN: "Interferon-γ and Hypoxia Priming Have Limited Effect on the miRNA Landscape of Human Mesenchymal Stromal Cells-Derived Extracellular Vesicles", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 8, 15 December 2020 (2020-12-15), pages 1 - 11, XP055948561, DOI: 10.3389/fcell.2020.581436
KIM HYEMEE, LEE MIN JOUNG, BAE EUN-HYE, RYU JIN SUK, KAUR GAGANDEEP, KIM HYEON JI, KIM JUN YEOB, BARREDA HEATHER, JUNG SUNG YOUN, : "Comprehensive Molecular Profiles of Functionally Effective MSC-Derived Extracellular Vesicles in Immunomodulation", MOLECULAR THERAPY, vol. 28, no. 7, 1 July 2020 (2020-07-01), US , pages 1628 - 1644, XP055948565, ISSN: 1525-0016, DOI: 10.1016/j.ymthe.2020.04.020
UPADHYA RAGHAVENDRA, MADHU LEELAVATHI N., ATTALURI SAHITHI, GITAÍ DANIEL LEITE GÓES, PINSON MARISA R, KODALI MAHEEDHAR, SHETTY GEE: "Extracellular vesicles from human iPSC‐derived neural stem cells: miRNA and protein signatures, and anti‐inflammatory and neurogenic properties", JOURNAL OF EXTRACELLULAR VESICLES, vol. 9, no. 1, 1 September 2020 (2020-09-01), UK , pages 1 - 21, XP055872955, ISSN: 2001-3078, DOI: 10.1080/20013078.2020.1809064
PELTZER JULIETTE, LUND KYLE, GORIOT MARIE-EMMANUELLE, GROSBOT MARION, LATAILLADE JEAN-JACQUES, MAUDUIT PHILIPPE, BANZET SÉBASTIEN: "Interferon-γ and Hypoxia Priming Have Limited Effect on the miRNA Landscape of Human Mesenchymal Stromal Cells-Derived Extracellular Vesicles", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 8, 15 December 2020 (2020-12-15), pages 1 - 11, XP055948561, DOI: 10.3389/fcell.2020.581436
KIM HYEMEE, LEE MIN JOUNG, BAE EUN-HYE, RYU JIN SUK, KAUR GAGANDEEP, KIM HYEON JI, KIM JUN YEOB, BARREDA HEATHER, JUNG SUNG YOUN, : "Comprehensive Molecular Profiles of Functionally Effective MSC-Derived Extracellular Vesicles in Immunomodulation", MOLECULAR THERAPY, vol. 28, no. 7, 1 July 2020 (2020-07-01), US , pages 1628 - 1644, XP055948565, ISSN: 1525-0016, DOI: 10.1016/j.ymthe.2020.04.020
UPADHYA RAGHAVENDRA, MADHU LEELAVATHI N., ATTALURI SAHITHI, GITAÍ DANIEL LEITE GÓES, PINSON MARISA R, KODALI MAHEEDHAR, SHETTY GEE: "Extracellular vesicles from human iPSC‐derived neural stem cells: miRNA and protein signatures, and anti‐inflammatory and neurogenic properties", JOURNAL OF EXTRACELLULAR VESICLES, vol. 9, no. 1, 1 September 2020 (2020-09-01), UK , pages 1 - 21, XP055872955, ISSN: 2001-3078, DOI: 10.1080/20013078.2020.1809064
Attorney, Agent or Firm:
ZHONGZI LAW OFFICE (CN)
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