Title:
MULTIPLE GENE EXPRESSION FOR ENGINEERING NOVEL PATHWAYS AND HYPEREXPRESSION OF FOREIGN PROTEINS IN PLANTS
Document Type and Number:
WIPO Patent Application WO2001064024
Kind Code:
B1
Abstract:
Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system.
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Inventors:
DANIELL HENRY (US)
MOAR WILLIAM (US)
MOAR WILLIAM (US)
Application Number:
PCT/US2001/006276
Publication Date:
November 01, 2001
Filing Date:
February 28, 2001
Export Citation:
Assignee:
UNIV AUBURN (US)
UNIV CENTRAL FLORIDA (US)
DANIELL HENRY (US)
MOAR WILLIAM (US)
UNIV CENTRAL FLORIDA (US)
DANIELL HENRY (US)
MOAR WILLIAM (US)
International Classes:
C07K14/325; C12N15/79; C12N15/82; (IPC1-7): A01H5/02; A01H5/10; A01H13/00; C12N1/20; C12N1/12; C12N5/04; C12N15/29; C12N15/32; C12N15/52; C12N15/31; C12N15/17; C12N15/13; C12N15/63; C12N15/74; C12N15/82
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