Title:
NOVEL CRISPR-ASSOCIATED TRANSPOSASE
Document Type and Number:
WIPO Patent Application WO/2022/242464
Kind Code:
A1
Abstract:
A novel CRISPR-associated transposase derived from pseudoalteromonas translucida KMM 520, wherein 16 PAMs can be identified and cargo genes can be inserted into 8 sites at a time with an efficiency of 100%, higher than a CRISPR-associated transposase derived from vibrio cholerae Tn6677; the transposition of the 15.4kb cargo gene to the corresponding target site has an efficiency of 100%, and the present transposase and the CRISPR-associated transposase derived from vibrio cholerae Tn6677 can be used in the same E. coli and function without interfering with one another, having broad prospects for application.
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Inventors:
YANG SHENG (CN)
YANG SIQI (CN)
ZHANG YIWEN (CN)
XU JIAQI (CN)
ZHANG JIAO (CN)
YANG SIQI (CN)
ZHANG YIWEN (CN)
XU JIAQI (CN)
ZHANG JIAO (CN)
Application Number:
PCT/CN2022/091102
Publication Date:
November 24, 2022
Filing Date:
May 06, 2022
Export Citation:
Assignee:
CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES (CN)
International Classes:
C12N15/113; C07K1/00; C12N9/22; C12N15/10; C12N15/90
Domestic Patent References:
| WO2020186262A1 | 2020-09-17 | |||
| WO2021016135A1 | 2021-01-28 |
Foreign References:
| US20200325474A1 | 2020-10-15 | |||
| CN112272704A | 2021-01-26 |
Other References:
YANG SIQI, ZHANG YIWEN, XU JIAQI, ZHANG JIAO, ZHANG JIEZE, YANG JUNJIE, JIANG YU, YANG SHENG: "Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 49, no. 17, 27 September 2021 (2021-09-27), GB , pages 10192 - 10202, XP093006464, ISSN: 0305-1048, DOI: 10.1093/nar/gkab752
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2466 [Pseudoalteromonas translucida KMM 520]", XP093006465, retrieved from NCBI Database accession no. ALS33555
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "putative transposase [Pseudoalteromonas translucida KMM 520]", XP093006466, retrieved from NCBI Database accession no. ALS33554
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2464 [Pseudoalteromonas translucida KMM 520]", XP093006472, retrieved from NCBI Database accession no. ALS33553
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2458 [Pseudoalteromonas translucida KMM 520]", XP093006478, retrieved from NCBI Database accession no. ALS33548
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2457 [Pseudoalteromonas translucida KMM 520]", XP093006483, retrieved from NCBI Database accession no. ALS33547
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2455 [Pseudoalteromonas translucida KMM 520]", XP093006488, retrieved from NCBI Database accession no. ALS33545
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2456 [Pseudoalteromonas translucida KMM 520]", XP093006494, retrieved from NCBI Database accession no. ALS33546
ZHANG YIWEN, SUN XIAOMAN, WANG QINGZHUO, XU JIAQI, DONG FENG, YANG SIQI, YANG JIAWEI, ZHANG ZIXU, QIAN YUAN, CHEN JUN, ZHANG JIAO,: "Multicopy Chromosomal Integration Using CRISPR-Associated Transposases", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 9, no. 8, 21 August 2020 (2020-08-21), Washington DC ,USA , pages 1998 - 2008, XP055901921, ISSN: 2161-5063, DOI: 10.1021/acssynbio.0c00073
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2466 [Pseudoalteromonas translucida KMM 520]", XP093006465, retrieved from NCBI Database accession no. ALS33555
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "putative transposase [Pseudoalteromonas translucida KMM 520]", XP093006466, retrieved from NCBI Database accession no. ALS33554
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2464 [Pseudoalteromonas translucida KMM 520]", XP093006472, retrieved from NCBI Database accession no. ALS33553
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2458 [Pseudoalteromonas translucida KMM 520]", XP093006478, retrieved from NCBI Database accession no. ALS33548
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2457 [Pseudoalteromonas translucida KMM 520]", XP093006483, retrieved from NCBI Database accession no. ALS33547
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2455 [Pseudoalteromonas translucida KMM 520]", XP093006488, retrieved from NCBI Database accession no. ALS33545
DATABASE PROTEIN 15 December 2015 (2015-12-15), ANONYMOUS : "hypothetical protein PTRA_a2456 [Pseudoalteromonas translucida KMM 520]", XP093006494, retrieved from NCBI Database accession no. ALS33546
ZHANG YIWEN, SUN XIAOMAN, WANG QINGZHUO, XU JIAQI, DONG FENG, YANG SIQI, YANG JIAWEI, ZHANG ZIXU, QIAN YUAN, CHEN JUN, ZHANG JIAO,: "Multicopy Chromosomal Integration Using CRISPR-Associated Transposases", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 9, no. 8, 21 August 2020 (2020-08-21), Washington DC ,USA , pages 1998 - 2008, XP055901921, ISSN: 2161-5063, DOI: 10.1021/acssynbio.0c00073
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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