Title:
PRIMER PROBE FOR DETECTION, PRIMER PROBE SET, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/125560
Kind Code:
A1
Abstract:
A primer probe for PCR detection, a primer probe set, and an application thereof. The primer probe and the primer probe set can simultaneously analyze the two dimensions of a fluorescent channel and a melting temperature in a single tube reaction. That is, by using a same fluorescent channel, different targets can be detected by means of a melting temperature characteristic; or different fluorescent channels are used, so that the target type detection of the product of the number of fluorescent channels and the melting temperature characteristic can be achieved. Moreover, a method for performing multiple PCR detection by using the primer probe has the advantages of being low in fluorescence background, adjustable in melting temperature, good in inclusiveness, and low in cost, having the single tube reaction, and being simple and convenient to operate, not easy to cause pollution, high in sensitivity, and wide in applicable range.
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Inventors:
ZHAO YUHANG (CN)
HU WANER (CN)
ZHAN HAOMIAO (CN)
TANG FANG (CN)
HU WANER (CN)
ZHAN HAOMIAO (CN)
TANG FANG (CN)
Application Number:
PCT/CN2022/142454
Publication Date:
July 06, 2023
Filing Date:
December 27, 2022
Export Citation:
Assignee:
MACCURA BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/70
Foreign References:
| CN110964814A | 2020-04-07 | |||
| CN108913759A | 2018-11-30 | |||
| CN110541033A | 2019-12-06 | |||
| CN112111566A | 2020-12-22 | |||
| JP2009148245A | 2009-07-09 |
Attorney, Agent or Firm:
YUHONG INTELLECTUAL PROPERTY LAW FIRM (CN)
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