Title:
RECOMBINANT ADENO-ASSOCIATED VIRUS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/222869
Kind Code:
A1
Abstract:
The present invention relates to a recombinant adeno-associated virus (AAV) and an application thereof. The present invention further relates to an AAV purification method and a purified AAV obtained thereby. The AAV in the present invention has organ or tissue targeting performance and/or high solid heart rate and/or can avoid neutralizing an antibody to a certain extent, such that the AAV can be used as a delivery vector of a therapeutic gene for the treatment of a related disease. The purification method in the present invention can change the target organ delivery capability of the AAV, and improve the liver transduction specificity of the AAV.
More Like This:
Inventors:
ZHAO KAI (CN)
CHEN CHEN (CN)
YANG CHEN (CN)
LIU QIANG (CN)
YUAN LONGHUI (CN)
DU ZENGMIN (CN)
JIANG WEI (CN)
WU XIA (CN)
ZHENG JING (CN)
XIAO XIAO (CN)
WANG LIQUN (CN)
WANG HUI (CN)
ZHAO YANG (CN)
CHEN CHEN (CN)
YANG CHEN (CN)
LIU QIANG (CN)
YUAN LONGHUI (CN)
DU ZENGMIN (CN)
JIANG WEI (CN)
WU XIA (CN)
ZHENG JING (CN)
XIAO XIAO (CN)
WANG LIQUN (CN)
WANG HUI (CN)
ZHAO YANG (CN)
Application Number:
PCT/CN2022/087162
Publication Date:
October 27, 2022
Filing Date:
April 15, 2022
Export Citation:
Assignee:
BELIEF BIOMED LTD (CN)
International Classes:
C07K14/015; A61K47/42; A61K48/00; A61P21/00; A61P27/02; C12N7/02; C12N15/35; C12N15/864
Foreign References:
| CN112961220A | 2021-06-15 | |||
| CN113121654A | 2021-07-16 | |||
| CN113121652A | 2021-07-16 | |||
| CN113121653A | 2021-07-16 | |||
| CN113121651A | 2021-07-16 | |||
| CN113121655A | 2021-07-16 | |||
| CN111655285A | 2020-09-11 |
Other References:
WANG YUQIU, YANG CHEN, HU HANYANG, CHEN CHEN, YAN MENGDI, LING FEIXIANG, WANG KATHY CHENG, WANG XINTAO, DENG ZHE, ZHOU XINYUE, ZHA: "Directed evolution of adeno-associated virus 5 capsid enables specific liver tropism", MOLECULAR THERAPY-NUCLEIC ACIDS, CELL PRESS, US, vol. 28, 1 June 2022 (2022-06-01), US , pages 293 - 306, XP055980992, ISSN: 2162-2531, DOI: 10.1016/j.omtn.2022.03.017
K VARADI, S MICHELFELDER, T KORFF, M HECKER, M TREPEL, H A KATUS, J A KLEINSCHMIDT, O J MÜLLER: "Novel random peptide libraries displayed on AAV serotype 9 for selection of endothelial cell-directed gene transfer vectors", GENE THERAPY, MACMILLAN PRESS LTD., vol. 19, no. 8, 1 August 2012 (2012-08-01), pages 800 - 809, XP055129104, ISSN: 09697128, DOI: 10.1038/gt.2011.143
.: "Scalable production and purification of adeno-associated virus (AAV) vector using the ExpiSf Expression System", APPLICATION NOTE - GIBCO, THERMO FISHER SCIENTIFIC, 31 December 2020 (2020-12-31), pages 1 - 5, XP055980999, Retrieved from the Internet [retrieved on 20221114]
YAN JINGHUI, ZOU YUEQI, LIU YUCUI: "RESEARCH ON SCREENING THE OPTIMAL ELUENT IN MONOCLONAL ANTIBODY AFFINITY CHROMATOGRAPHY", CHINESE MASTER’S THESES FULL-TEXT DATABASE, AGRICULTURAL SCIENCE AND TECHNOLOGY, vol. 4, 31 December 1991 (1991-12-31), pages 47 - 51, XP055981001, ISSN: 1001-9383, DOI: 10.16191/j.cnki.hbkx.1991.04.009
K VARADI, S MICHELFELDER, T KORFF, M HECKER, M TREPEL, H A KATUS, J A KLEINSCHMIDT, O J MÜLLER: "Novel random peptide libraries displayed on AAV serotype 9 for selection of endothelial cell-directed gene transfer vectors", GENE THERAPY, MACMILLAN PRESS LTD., vol. 19, no. 8, 1 August 2012 (2012-08-01), pages 800 - 809, XP055129104, ISSN: 09697128, DOI: 10.1038/gt.2011.143
.: "Scalable production and purification of adeno-associated virus (AAV) vector using the ExpiSf Expression System", APPLICATION NOTE - GIBCO, THERMO FISHER SCIENTIFIC, 31 December 2020 (2020-12-31), pages 1 - 5, XP055980999, Retrieved from the Internet
YAN JINGHUI, ZOU YUEQI, LIU YUCUI: "RESEARCH ON SCREENING THE OPTIMAL ELUENT IN MONOCLONAL ANTIBODY AFFINITY CHROMATOGRAPHY", CHINESE MASTER’S THESES FULL-TEXT DATABASE, AGRICULTURAL SCIENCE AND TECHNOLOGY, vol. 4, 31 December 1991 (1991-12-31), pages 47 - 51, XP055981001, ISSN: 1001-9383, DOI: 10.16191/j.cnki.hbkx.1991.04.009
Attorney, Agent or Firm:
WATSON & BAND INTELLECTUAL PROPERTY AGENT LTD. (CN)
Download PDF: