Title:
RECOMBINANT YEAST AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/169200
Kind Code:
A1
Abstract:
Provided are a recombinant yeast for producing lacto-N-triose, and an application thereof. The recombinant yeast comprises an amino acid sequence of a β-N-acetylglucosaminyl transferase and a coding nucleic acid sequence thereof, and/or a disruption of a marker gene.
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Inventors:
FANG XU (CN)
LI FENGYI (CN)
HAN LIJUAN (CN)
YIN WENCHENG (CN)
LI FENGYI (CN)
HAN LIJUAN (CN)
YIN WENCHENG (CN)
Application Number:
PCT/CN2023/077280
Publication Date:
September 14, 2023
Filing Date:
February 21, 2023
Export Citation:
Assignee:
SHANDONG HENGLU BIOTECH CO LTD (CN)
International Classes:
C12N1/19; C12N9/10; C12N9/38; C12N15/54; C12N15/56; C12P7/06; C12P19/18; C12P19/26; C12R1/645
Foreign References:
| CN112280727A | 2021-01-29 | |||
| CN111979168A | 2020-11-24 | |||
| US20140178933A1 | 2014-06-26 | |||
| CN1840665A | 2006-10-04 | |||
| CN110628842A | 2019-12-31 | |||
| CN102834509A | 2012-12-19 |
Other References:
DATABASE Protein 17 May 1996 (1996-05-17), ANONYMOUS: "glycosyl tranferase [Neisseria meningitidis]", XP093090882, retrieved from NCBI Database accession no. AAC44084.1
DATABASE Protein 9 October 2017 (2017-10-09), ANONYMOUS: "glycosyltransferase family 8 protein [Helicobacter pylori]", XP093090884, retrieved from NCBI Database accession no. WP_000199766.1
DATABASE Protein 25 June 2019 (2019-06-25), ANONYMOUS: "glycosyltransferase family 2 protein, partial [Salmonella enterica]", XP093090887, retrieved from NCBI Database accession no. WP_140239824.1
ZHU YINGYING, WAN LI, MENG JIAWEI, LUO GUOCONG, CHEN GENG, WU HAO, ZHANG WENLI, MU WANMENG: "Metabolic Engineering of Escherichia coli for Lacto- N -triose II Production with High Productivity", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 12, 31 March 2021 (2021-03-31), US , pages 3702 - 3711, XP093090888, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c00246
DATABASE Protein 9 October 2017 (2017-10-09), ANONYMOUS: "glycosyltransferase family 8 protein [Helicobacter pylori]", XP093090884, retrieved from NCBI Database accession no. WP_000199766.1
DATABASE Protein 25 June 2019 (2019-06-25), ANONYMOUS: "glycosyltransferase family 2 protein, partial [Salmonella enterica]", XP093090887, retrieved from NCBI Database accession no. WP_140239824.1
ZHU YINGYING, WAN LI, MENG JIAWEI, LUO GUOCONG, CHEN GENG, WU HAO, ZHANG WENLI, MU WANMENG: "Metabolic Engineering of Escherichia coli for Lacto- N -triose II Production with High Productivity", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 69, no. 12, 31 March 2021 (2021-03-31), US , pages 3702 - 3711, XP093090888, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.1c00246
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