Title:
SYNTHETIC MOLECULES FOR LABELING HISTIDINE-RICH PROTEINS
Document Type and Number:
WIPO Patent Application WO2004065548
Kind Code:
A3
Abstract:
The invention provides Zn-chelating compounds that are molecularly engineered to bind to a specific target sequence in a protein of interest. The Zn<2+> ion is far less toxic and promiscuous than nickel and therefore provides an attractive alternative to Ni-based labeling systems. Invention Zn-chelating compounds also do not require oxidizable thiols and therefore can be used in non-reducing environments such as the surface of living cells. In addition, the target sequence is genetically encodable and requires incorporation of only a few amino acids, unlike fusions to fluorescent proteins such as GFP.
Inventors:
TSIEN ROGER Y (US)
HAUSER CHRISTINA (DE)
HAUSER CHRISTINA (DE)
Application Number:
PCT/US2004/001230
Publication Date:
April 28, 2005
Filing Date:
January 16, 2004
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
TSIEN ROGER Y (US)
HAUSER CHRISTINA (DE)
TSIEN ROGER Y (US)
HAUSER CHRISTINA (DE)
International Classes:
C07D405/12; (IPC1-7): A61K31/352; A61K31/4433; A61K31/5375; C07D265/30; C07D311/80; C07D413/14
Foreign References:
| US5262333A | 1993-11-16 |
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