Title:
TAG PROTEIN FOR INCREASING WATER SOLUBILITY AND HEAT STABILITY OF TARGET PROTEIN, AND FUSION PROTEIN COMPRISING SAME
Document Type and Number:
WIPO Patent Application WO/2021/054774
Kind Code:
A1
Abstract:
The present invention relates to a fusion protein in which a target protein and an Oct-1 protein-containing protein tag are linked, an expression structure comprising a nucleotide sequence encoding same, a recombinant vector including same, and a transformed cell including same.
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Inventors:
KWEON DAE-HYUK (KR)
PARK YUN-JEONG (KR)
JEONG KI-JUN (KR)
BAE YOON-JIN (KR)
PARK YUN-JEONG (KR)
JEONG KI-JUN (KR)
BAE YOON-JIN (KR)
Application Number:
PCT/KR2020/012640
Publication Date:
March 25, 2021
Filing Date:
September 18, 2020
Export Citation:
Assignee:
RESEARCH & BUSINESS FOUND SUNGKYUNKWAN UNIV (KR)
KOREA ADVANCED INST SCI & TECH (KR)
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C07K14/47; C12N9/02; C12N9/10
Foreign References:
| KR20070019340A | 2007-02-15 | |||
| KR20040059490A | 2004-07-05 |
Other References:
PARK YUNJEONG: "Stabilization of proteins inside cells by immobilization on plasmid", MASTER'S THESIS, SUNGKYUNKWAN UNIVERSITY, 1 August 2019 (2019-08-01), Korea, pages 1 - 40, XP009526836
PARK JONG HYUN, HYUK WOONG KWON, KI JUN JEONG: "Development of a plasmid display system with an Oct-1 DNA-binding domain suitable for in vitro screening of engineered proteins", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 116, no. 2, pages 246 - 252, XP055793650, DOI: 10.1016/j.biosc.2013.02.005
PARK YUNJEONG, SHIN JONGHYEOK, YANG JINKYEONG, KIM HOOYEON, JUNG YOUNGHUN, OH HYUNSEOK, KIM YONGJOON, HWANG JAEHYEON, PARK MYEONGS: "Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, XP055793653, DOI: 10.3389/fbioe.2019.00444
PARK JONG HYUN, HYUK WOONG KWON, KI JUN JEONG: "Development of a plasmid display system with an Oct-1 DNA-binding domain suitable for in vitro screening of engineered proteins", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 116, no. 2, pages 246 - 252, XP055793650, DOI: 10.1016/j.biosc.2013.02.005
PARK YUNJEONG, SHIN JONGHYEOK, YANG JINKYEONG, KIM HOOYEON, JUNG YOUNGHUN, OH HYUNSEOK, KIM YONGJOON, HWANG JAEHYEON, PARK MYEONGS: "Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, XP055793653, DOI: 10.3389/fbioe.2019.00444
Attorney, Agent or Firm:
KONG, Byung Wook (KR)
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