Title:
VIRUS NUCLEIC ACID SAMPLE DILUENT, VIRUS NUCLEIC ACID SAMPLE EXTRACTION KIT, AND VIRUS NUCLEIC ACID EXTRACTION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/066120
Kind Code:
A1
Abstract:
Provided are a virus nucleic acid sample diluent, a virus nucleic acid sample extraction kit, and a virus nucleic acid extraction method. The provided virus nucleic acid sample release agent can be compatible with various sampling sets. Meanwhile, the critical micelle concentration of a used surfactant is reduced to the maximum extent by means of adjusting the types and proportions of components. The lysis of a cell and a pathogen can be ensured, and the inhibitory effect on a subsequent detection system can also be reduced. Under the condition that an ion exchange resin is added, a protein and metal ions, such as calcium and magnesium ions, that affect a subsequent reaction can be adsorbed from a sample, and cell and virus lysis can also be promoted, such that the compatibility of the system is enhanced. The detection system has good compatibility, can be compatible with various qPCR detection systems at the same time, and can also be used for an isothermal RAA amplification system. The present invention is not only suitable for a liquid detection system, but also can be used for a dry powder detection system with a large sample volume.
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Inventors:
SHEN ZIMING (CN)
ZHAO BAIHUI (CN)
LI CHUNYAN (CN)
ZHANG CHUNLEI (CN)
ZHAO BAIHUI (CN)
LI CHUNYAN (CN)
ZHANG CHUNLEI (CN)
Application Number:
PCT/CN2022/144219
Publication Date:
April 04, 2024
Filing Date:
December 30, 2022
Export Citation:
Assignee:
SHANGHAI BIOGERM MEDICAL TECH CO LTD (CN)
International Classes:
C12N15/10; C12Q1/6806; C12R1/92
Domestic Patent References:
| WO2021229066A1 | 2021-11-18 |
Foreign References:
| CN115386575A | 2022-11-25 | |||
| CN114591945A | 2022-06-07 | |||
| CN114181932A | 2022-03-15 | |||
| US20200149120A1 | 2020-05-14 | |||
| CN103642919A | 2014-03-19 | |||
| US20180235206A1 | 2018-08-23 | |||
| CN113985027A | 2022-01-28 | |||
| CN112322797A | 2021-02-05 |
Other References:
YAN LIU: "Development of rapid extraction reagent for DNA release of foodborne pathogenic bacteria", CHINESE JOURNAL OF FOOD HYGIENE, SCHOOL OF CHINESE MATERIA MEDICA, GUANGZHOU UNIVERSITY OF CHINESE MEDICINE, GUANGZHOU, CHINA, CN, vol. 35, no. 1, 11 October 2022 (2022-10-11), CN , pages 49 - 54, XP093152362, ISSN: 1004-8456, DOI: 10.13590/j.cjfh.2023.01.008
ZHANG JUNGE: "Influence of different rapid one-step DNA extraction on PCR detection of Mycoplasma pneumoniae", DISEASE SURVEILLANCE, vol. 33, no. 6, 30 June 2018 (2018-06-30), pages 503 - 509, XP093152364, DOI: 10.3784/j.issn.1003-9961.2018.06.014
ZHANG JUNGE: "Influence of different rapid one-step DNA extraction on PCR detection of Mycoplasma pneumoniae", DISEASE SURVEILLANCE, vol. 33, no. 6, 30 June 2018 (2018-06-30), pages 503 - 509, XP093152364, DOI: 10.3784/j.issn.1003-9961.2018.06.014
Attorney, Agent or Firm:
CHOFN INTELLECTUAL PROPERTY (CN)
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