GENETICALLY ENGINEERED STRAIN CAPABLE OF PRODUCING L-ALANINE, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF

Title:

GENETICALLY ENGINEERED STRAIN CAPABLE OF PRODUCING L-ALANINE, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2023/056699

Kind Code:

A1

Abstract:

Disclosed are a genetically engineered strain capable of producing L-alanine, a construction method therefor, and an application thereof. A genetically engineered strain capable of efficiently producing alanine at a high temperature of 42°C to 55°C can be constructed by enhancing a glycolysis pathway or/and introducing a thermostable alanine dehydrogenase gene. The optical purity of L-alanine can further be significantly improved by knocking out an alanine racemase gene. When a lactic acid synthesis pathway exists in starting bacteria, the proportion of a pyruvic acid synthesis pathway can be increased by blocking the lactic acid synthesis pathway, so as to further improve the yield of L-alanine, such that high-temperature fermentation production of L-alanine at 42°C-55°C is achieved, and the yield of alanine can reach more than 95 g/L.

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Inventors:

XU PING (CN)
HAN XIAO (CN)
TAO FEI (CN)
MU XIAOLING (CN)
CHEN SIHONG (CN)
LI WEILI (CN)

Application Number:

PCT/CN2021/135643

Publication Date:

April 13, 2023

Filing Date:

December 06, 2021

Export Citation:

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Assignee:

ANHUI BBCA BIOCHEMICAL CO LTD (CN)

International Classes:

C12N15/64; C12N1/21; C12N15/75; C12P13/06; C12R1/07; C12R1/10

Domestic Patent References:

WO2008119009A2

2008-10-02

Foreign References:

CN104774790A	2015-07-15
CN105907732A	2016-08-31
CN105899664A	2016-08-24
CN101974476A	2011-02-16
CN104593405A	2015-05-06

Other References:

YAMAMOTO SHOGO, GUNJI WATARU, SUZUKI HIROAKI, TODA HIROSHI, SUDA MASAKO, JOJIMA TORU, INUI MASAYUKI, YUKAWA HIDEAKI: "Overexpression of Genes Encoding Glycolytic Enzymes in Corynebacterium glutamicum Enhances Glucose Metabolism and Alanine Production under Oxygen Deprivation Conditions", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 78, no. 12, 15 June 2012 (2012-06-15), US , pages 4447 - 4457, XP093055452, ISSN: 0099-2240, DOI: 10.1128/AEM.07998-11

Attorney, Agent or Firm:

SUNRAY INTELLECTUAL PROPERTY ATTORNEYS (CN)

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