Title:
METHOD FOR PRODUCING OLIGODENDROCYTE PRECURSOR CELL FROM PLURIPOTENT STEM CELL
Document Type and Number:
WIPO Patent Application WO/2013/012269
Kind Code:
A3
Abstract:
The present invention relates to a method for producing an oligodendrocyte precursor cell from a pluripotent stem cell and a neural precursor cell produced by the method, and more specifically, to a method comprising the steps of: (a) obtaining a cell population including the neural precursor cell by differentiating the pluripotent stem cell into a neural rosette; (b) putting an A2B5-specifically binding antibody into contact with the cell population; (c) separating an A2B5 positive cell to which the antibody to A2B5 is bound; and (d) differentiating the A2B5 positive cell into the oligodendrocyte precursor cell. According to the present invention, a more efficient and stable method for producing the oligodentrocyte precursor cell compared to existing methods can be established, and indicators which are appropriate for tracing a differentiation process can be provided. Also, the disadvantage of not maintaining a constant differentiation rate due to a variable during a differentiation process can be overcome, and a more pure production method can be provided by shortening the differentiation process.
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Inventors:
KIM DONG-WOOK (KR)
KIM DAE-SUNG (KR)
LIM BOYOUNG (KR)
KIM DAE-SUNG (KR)
LIM BOYOUNG (KR)
Application Number:
PCT/KR2012/005775
Publication Date:
April 11, 2013
Filing Date:
July 19, 2012
Export Citation:
Assignee:
UNIV YONSEI IACF (KR)
KIM DONG-WOOK (KR)
KIM DAE-SUNG (KR)
LIM BOYOUNG (KR)
KIM DONG-WOOK (KR)
KIM DAE-SUNG (KR)
LIM BOYOUNG (KR)
International Classes:
C12N5/0735; A61K35/30; A61K35/56; A61P25/00; A61K35/545
Other References:
OGAWA, S-I. ET AL.: "Immunopanning selection of A2B5-positive cells increased the differentiation efficiency of induced pluripotent stem cells into oligodendrocytes", NEUROSCIENCE LETTERS, vol. 489, no. 2, 4 February 2011 (2011-02-04), pages 79 - 83
ZHANG, S-C. ET AL.: "In vitro differentiation of transplantable neural precursors from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 19, no. 12, 2001, pages 1129 - 1133
NUNES, MC. ET AL.: "Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain", NATURE MEDICINE, vol. 9, no. 4, April 2003 (2003-04-01), pages 439 - 447
ABRANCHES, E. ET AL.: "Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo", PLOS ONE, vol. 4, no. 7, 21 July 2009 (2009-07-21), pages E6286
ELKABETZ, Y. ET AL.: "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage", GENES & DEVELOPMENT, vol. 22, no. 2, 15 January 2008 (2008-01-15), pages 152 - 165
ZHANG, S-C. ET AL.: "In vitro differentiation of transplantable neural precursors from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 19, no. 12, 2001, pages 1129 - 1133
NUNES, MC. ET AL.: "Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain", NATURE MEDICINE, vol. 9, no. 4, April 2003 (2003-04-01), pages 439 - 447
ABRANCHES, E. ET AL.: "Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo", PLOS ONE, vol. 4, no. 7, 21 July 2009 (2009-07-21), pages E6286
ELKABETZ, Y. ET AL.: "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage", GENES & DEVELOPMENT, vol. 22, no. 2, 15 January 2008 (2008-01-15), pages 152 - 165
Attorney, Agent or Firm:
YANG, Boo-Hyun (1659-2 Inhundong Gwanak-gu, Seoul 151-832, KR)
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