Title:
TECHNOLOGY FOR HIGH-PURITY REFINEMENT OF Gc PROTEIN
Document Type and Number:
WIPO Patent Application WO/2021/192859
Kind Code:
A1
Abstract:
The present invention provides an improved method for high-purity refinement of Gc protein, and a method for producing GcMAF. According to the present invention, by refining Gc protein using a combination of affinity chromatography and anion exchange chromatography, it is possible to perform high-purity refinement of Gc protein as compared to methods using hitherto known technologies in which refinement is performed by using affinity chromatography alone. Accordingly, it is possible to efficiently produce GcMAF.
More Like This:
Inventors:
TAKAHASHI SACHIKO (JP)
UEDA MASUMI (JP)
MORITA YUKI (JP)
TANOKURA MASARU (JP)
MIYAKAWA TAKUYA (JP)
LI XUYANG (JP)
MURAMATSU TOMONARI (JP)
WANG RONG (JP)
UEDA MASUMI (JP)
MORITA YUKI (JP)
TANOKURA MASARU (JP)
MIYAKAWA TAKUYA (JP)
LI XUYANG (JP)
MURAMATSU TOMONARI (JP)
WANG RONG (JP)
Application Number:
PCT/JP2021/008050
Publication Date:
September 30, 2021
Filing Date:
March 03, 2021
Export Citation:
Assignee:
KK MEDICALBIARA (JP)
International Classes:
C07K1/22; C07K1/18; C07K14/47; C12P1/00; C12P21/00
Domestic Patent References:
| WO2019117295A1 | 2019-06-20 |
Foreign References:
| JP2012122038A | 2012-06-28 | |||
| US20030036638A1 | 2003-02-20 | |||
| JPH06510908A | 1994-12-08 |
Other References:
WBINSON, RABERT C ET AL.: "Stabilization of the structure of horse plasma vitamin D binding protein by disulfide bonds", BIOCHEM. CELL BIOL, vol. 70, 1992, pages 10 - 15
CHAPUIS-CELLIER, C. ; GIANAZZA, E. ; ARNAUD, P.: "Interaction of group-specific component (vitamin D-binding protein) with immobilized cibacron blue F3-GA", BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,, vol. 709, no. 2, 20 December 1982 (1982-12-20), AMSTERDAM; NL , pages 353 - 357, XP025210589, ISSN: 0167-4838, DOI: 10.1016/0167-4838(82)90478-2
MORITA YUKI, WANG RONG, LI XUYANG, MURAMATSU TOMONARI, UEDA MASUMI, HACHIMURA SATOSHI, TAKAHASHI SACHIKO, MIYAKAWA TAKUYA, TANOKUR: "Improved preparation of group-specific component (Gc) protein to derive macrophage activating factor", PROTEIN EXPRESSION AND PURIFICATION, vol. 175, 105714, 1 November 2020 (2020-11-01), SAN DIEGO, CA. , XP055861289, ISSN: 1046-5928, DOI: 10.1016/j.pep.2020.105714
CHAPUIS-CELLIER, C. ; GIANAZZA, E. ; ARNAUD, P.: "Interaction of group-specific component (vitamin D-binding protein) with immobilized cibacron blue F3-GA", BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,, vol. 709, no. 2, 20 December 1982 (1982-12-20), AMSTERDAM; NL , pages 353 - 357, XP025210589, ISSN: 0167-4838, DOI: 10.1016/0167-4838(82)90478-2
MORITA YUKI, WANG RONG, LI XUYANG, MURAMATSU TOMONARI, UEDA MASUMI, HACHIMURA SATOSHI, TAKAHASHI SACHIKO, MIYAKAWA TAKUYA, TANOKUR: "Improved preparation of group-specific component (Gc) protein to derive macrophage activating factor", PROTEIN EXPRESSION AND PURIFICATION, vol. 175, 105714, 1 November 2020 (2020-11-01), SAN DIEGO, CA. , XP055861289, ISSN: 1046-5928, DOI: 10.1016/j.pep.2020.105714
Attorney, Agent or Firm:
EINSEL Felix-Reinhard et al. (JP)
Download PDF: